An Empirical Analysis of Work Overload, Organizational Commitment and Turnover Intentions among Employees of Banking Sector

The work overload is often considered as the most critical factor which effects the employees’ commitment and turnover intention toward organization, therefore, the current study aims to evaluate effects of Work Overload (WO) on Organizational Commitment (OC) and Turnover Intentions (TOI) of employees. The data was collected from 296 employees of selected banks.  The data was assessed through Pearson Correlation and Regression equation. It was found that work overload negatively influenced organizational commitment while positively affects employees’ turnover intentions. The findings of this study can help the decision makers to effectively manage employees’ workload and to improve organizational commitment and reduce employees’ turnover intentions. Such type of behavioral outcomes has the potential to augment organizational performance

Gastroprotective effects of aquous solution: Acacia catechu on gastric mucosal injury in experimental albino rats model

Background: Around the globe, acid peptic disease and its potential complications are among the major cause of morbidity and is a significant burden on health system. Till now, the main stay of treatment are the pharmaceutical agents which decrease the secretion of acid. But these have potential complications and tolerability issues. Among such plants one is Acacia catechu commonly available in India and Pakistan.Methods: This experimental study involved 48 albino rats that were divided into four groups. Group A, B, C, and D were given water, 100mg aspirin, 100 mg aspirin+ 250 mg Acacia catechu and 100 mg aspirin+500 mg Aacacia catechu respectively, and were sacrificed on day 3, 7 and 14th day of study and observed for changes.Results: Difference between blood vessels in subgroup B1 and subgroup C1 with subgroup D1 was statistically significant (p=0.05) and (p=0.001). Group C1 and D1 had normal vessels in mucosa and submucosa. However, dilatation of blood vessels was noted in both groups receiving Acacia catechu along with ASA.Conclusions: Aqueous extracts of Acacia catechu stem and bark showed significant anti-ulcer and anti-inflammatory activities by increasing blood flow to the stomach

Management of Adenovirus in Children after Allogeneic Hematopoietic Stem Cell Transplantation

Adenovirus (ADV) can cause significant morbidity and mortality in children following haematopoietic stem cell transplantation (HSCT), with an incidence of up to 27% and notable associated morbidity and mortality. T-cell depleted grafts and severe lymphopenia are major risk factors for the development of adenovirus disease after HSCT. Current antiviral treatments are at best virostatic and may have significant side effects. Adoptive transfer of donor-derived virus-specific T cells has been shown to be an effective strategy for the prevention and treatment of ADV infection after HSCT. Here we review progress in the field and present a pathway for the management of adenovirus in the posttransplant setting

GEOFIZIČKA ISTRAŽIVANJA POTENCIJALNOGA KLIZIŠTA U PODRUČJU MAYOON, DISTRIKT HUNZA, GILGIT-BALTISTAN, PAKISTAN

The Mayoon landslide in the Hunza District is a slowly developed, non-catastrophic landslide that has gained its importance in the last few years after its rapid activation and fast slip rate. The area is characterized by high earthquake hazards (zone 3 with a peak ground acceleration value of 2.4–3.2 m/s2) by the Building Code of Pakistan due to frequent earth quakes. The past high earthquake activity in the area has displaced the foliated rocks towards the south and is responsible for opening the bedrock joints. The head and body of the landslide are covered by unconsolidated material and have fractures of varying lengths and widths. The non-invasive geophysical techniques, including Ground Penetrating Radar (GPR) and Electrical Resistivity Soundings (ERS), are deployed to evaluate the Mayoon landslide subsurface. The subsurface is interpreted into a two-layer model. Bright reflectors and highly variable resistivity characterize the top layer (Layer-1). This layer is associated with a loose, highly heterogeneous, fragmented material deposited under glacial settings over the existing bedrock. Hyperbolic reflections and intermediate resistivity characterize the bottom layer (Layer-2). This layer is associated with foliated metamorphic bedrock. The hyperbolic reflections show faults/fractures within the bedrock. The extension of these fractures/faults with depth is uncertain due to decay in the GPR signal with depth. The intermediate resistivity shows the bedrock is weathered and foliated. Reflections within Layer-1 have disrupted directly above the fractures/faults suggesting a possible movement. A bright reflection between the two layers highlights the presence of the debonded surface. Loose material within Layer-1 coupled with debonding possesses a significant hazard to generate a landslide under unfavourable conditions, such as an intense rainstorm or earthquake activity.Klizište Mayoon u distriktu Hunza pripada skupini sporo razvijajućih, nekatastrofičnih klizišta. Važnost mu je porasla u nekoliko zadnjih godina nakon što se klizanje znatno ubrzalo. Cijelo područje obilježeno je visokim potresnim rizikom (zona 3, s najvećim ubrzanjem tla 2,4 – 3,2 m/s2), a u skladu s pakistanskim propisima o gradnji. Snažni potresi u prošlosti pomaknuli su blokove metamorfnih stijena prema jugu i uzrokovali otvaranje brojnih pukotina u stijenama podine. Čelo i tijelo klizišta prekriveno je nekonsolidiranim materijalom te ima pukotine različitih širina i dužina. Prostor klizišta istražen je neinvazivnim geofizičkim tehnikama poput georadara i mjerenja električne otpornosti. Podzemlje je prikazano dvoslojnim modelom. Prvi sloj (1) predstavljen je snažnim reflektorom i vrlo promjenjivom otpornošću. Sastavljen je od rastresitih, vrlo heterogenih, fragmentiranih materijala taloženih tijekom glacijala preko stijenske podine. Ispod je drugi sloj (2) obilježen hiperboličkim refleksima te umjerenom otpornošću i pruža se unutar folijacijskih, metamorfnih stijena. Oblik refleksa upozorava na rasjede i pukotine podine, no oni se teško prate s povećanjem dubine. Otpornost također upućuje na trošenje i folijaciju. Refleksi u sloju 1 prekidaju se iznad rasjeda i pukotina upozoravajući na moguće gibanje. Snažni refleksi između dvaju slojeva naglašavaju postojanje granice između njih. Rastresiti materijal u sloju 1, zajedno s postojanjem takve granice, predstavlja znatan rizik nastanka klizišta, posebice u rizičnim uvjetima poput olujnoga pljuska ili potresa

Human involucrin promoter mediates repression-resistant and compartment-specific LEKTI expression

Gene-modified skin grafts, produced through gene transfer to human keratinocyte stem cells, offer the possibility of therapeutic benefit for inherited skin diseases. We have previously described efficient lentiviral vector–mediated gene transfer to keratinocyte stem cells and the generation of human skin grafts for the inherited skin disease, Netherton syndrome, which arises due to mutations in serine protease inhibitor Kazal-type 5 (SPINK5). Vectors incorporating an internal murine retroviral–derived promoter [spleen focus-forming virus (SFFV)] in combination with a codon-optimized SPINK5 transgene supported high levels of reconstitution and robust correction of skin architecture. Subsequent longer-term experiments have uncovered unanticipated silencing phenomena, with loss of SPINK5 gene expression over time. The inadvertent introduction of CpG sites during codon optimization appears to have rendered vectors susceptible to silencing due to methylation across the promoter–transgene boundary. Substitution of the methylation-susceptible SFFV promoter with a 572-bp minimal human involucrin promoter (INVOp), which encodes very few CpG sites, prevented repression of the SPINK5 transgene and resulted in durable and highly compartment-specific reconstitution of lympho-epithelial Kazal-type–related inhibitor (LEKTI) in human skin grafted onto immunodeficient mice.We conclude that skin grafts modified with lentiviral vectors encoding INVOp offer a suitable platform for therapeutic gene therapy in Netherton syndrome, and our experience highlights unanticipated effects of transgene codon optimization

Highly Efficient Visible Hologram through Dielectric Metasurface

To achieve applied aspect of metasurfaces in the visible regime, dielectric materials with low absorption are indispensable. This work presents highly efficient generation of hologram via processed amorphous silicon, which exhibits significantly low absorption in the region of interest. The phase and the polarization of transmitted light are tailored by varying the orientation of dielectric nanorods whereas their conversion efficiency is optimized by adjusting their structural parameters. Better image fidelity and higher conversion efficiency (up-to 75%) are achieved as compared to previously reported work. The proposed design methodology paves a way toward on-chip realization of various novel phenomena with substantially enhanced performance.11Yscopu

Moderating Role of Organizational Justice between Emotional Exhaustion and Job-related Outcomes: Longitudinal Study at Education Sector

Previous studies anticipated that there is need of justice in educational institutions in order to protect the academicians’ psychological as well as emotional needs under teaching profession. Therefore, the purpose of current study is to examine the moderating role of organizational justice between the relationship of emotional exhaustion and job related outcomes (Organizational commitment and Organizational loyalty).In order to accomplish this purpose, the survey questionnaire method was used. The study is quantitative, descriptive and longitudinal in nature. 250 respondents were selected in time1 & time2 by applying simple random sampling. Results indicated that emotional exhaustion and employee loyalty are negatively associated with each other (Bt1= -.328; Tr2= -.329). Similarly negative correlation found between emotional exhaustion and commitment (Tr1= -.425; Tr2= -.427).Results in time2 exhibited more understanding about understudy relationships. Moreover, results also enlightened that organizational justice significantly moderate on the relationship of emotional exhaustion and job related outcomes (Organizational commitment and Organizational loyalty)

Autologous transplantation of amniotic fluid-derived mesenchymal stem cells into sheep fetuses

Long-term engraftment and phenotype correction has been difficult to achieve in humans after in utero stem cell transplantation mainly because of allogeneic rejection. Autologous cells could be obtained during gestation from the amniotic fluid with minimal risk for the fetus and the mother. Using a sheep model, we explored the possibility of using amniotic fluid mesenchymal stem cells (AFMSCs) for autologous in utero stem cell/gene therapy. We collected amniotic fluid (AF) under ultrasound-guided amniocentesis in early gestation pregnant sheep (n = 9, 58 days of gestation, term = 145 days). AFMSCs were isolated and expanded in all sampled fetal sheep. Those cells were transduced using an HIV vector encoding enhanced green fluorescent protein (GFP) with 63.2% (range 38.3-96.2%) transduction efficiency rate. After expansion, transduced AFMSCs were injected into the peritoneal cavity of each donor fetal sheep at 76 days under ultrasound guidance. One ewe miscarried twin fetuses after amniocentesis. Intraperitoneal injection was successful in the remaining 7 fetal sheep giving a 78% survival for the full procedure. Tissues were sampled at postmortem examination 2 weeks later. PCR analysis detected GFP-positive cells in fetal tissues including liver, heart, placenta, membrane, umbilical cord, adrenal gland, and muscle. GFP protein was detected in these tissues by Western blotting and further confirmed by cytofluorimetric and immunofluorescence analyses. This is the first demonstration of autologous stem cell transplantation in the fetus using AFMSCs. Autologous cells derived from AF showed widespread organ migration and could offer an alternative way to ameliorate prenatal congenital diseas

Non-osteopenic Bone Pathology After Allo-hematopoietic Stem Cell Transplantation in Patients with Inborn Errors of Immunity

PURPOSE: There is a lack of data on post-HSCT non-osteopenic bone pathology specifically for children with inborn errors of immunity (IEI). We collected data on non-osteopenic bone pathology in children with IEI post-HSCT over two decades in a large tertiary pediatric immunology center. METHODS: Descriptive study with data analysis of bone pathology in allo-HSCT for IEI was performed between 1/1/2000 to 31/12/2018 including patients alive at follow-up to July 2022. Records were analyzed for bone pathology and risk factors. Exclusion criteria included isolated reduced bone density, fractures, and skeletal anomalies due to underlying IEI and short stature without other bone pathology. Bone pathologies were divided into 5 categories: bone tumors; skeletal dysplasia; avascular necrosis; evolving bone deformities; slipped upper femoral epiphysis. RESULTS: A total of 429 children received HSCT between 2000 and 2018; 340 are alive at last assessment. Non-osteopenic bone pathology was observed post-HSCT in 9.4% of patients (32/340, mean 7.8 years post-HSCT). Eleven patients (34%) had > 1 category of bone pathology. Seventeen patients (17/32; 53%) presented with bilateral bone pathology. The majority of patients received treosulfan-based conditioning (26/32; 81.2%). Totally, 65.6% (21/32) of patients had a history of prolonged steroid use (> 6 months). Pain was the presenting symptom in 66% of patients, and surgical intervention was required in 43.7%. The highest incidence of bone pathologies was seen in Wiskott-Aldrich syndrome (WAS) (n = 8/34; 23.5%) followed by hemophagocytic lymphohistiocytosis patients (n = 3/16; 18.8%). CONCLUSION: Non-osteopenic bone pathology in long-term survivors of allo-HSCT for IEI is not rare. Most patients did not present with complaints until at least 5 years post-HSCT highlighting the need for ongoing bone health assessment for patients with IEI. Children presenting with stunted growth and bone pathology post-HSCT should undergo skeletal survey to rule out development of post-HSCT skeletal dysplasia. Increased rates and complexity of bone pathology were seen amongst patients with Wiskott-Aldrich syndrome